Phanta Max Super-Fidelity DNA Polymerase (P505)
Introduction
Phanta Max Super-Fidelity DNA Polymerase is a highly economical and accurate enzyme made for programs that involve higher-fidelity DNA amplification. This polymerase is ideal for use in PCR, cloning, as well as other molecular biology strategies the place precision and trustworthiness are paramount.
Vital Functions
1. Super-Fidelity
Overview: Phanta Max Super-Fidelity DNA Polymerase provides Extraordinary accuracy, drastically cutting down the mistake fee all through DNA amplification.
Gain: This superior-fidelity function is vital for programs for example cloning, mutagenesis, and sequencing, the place even slight problems can lead to sizeable concerns.
2. High Performance
Overview: The enzyme exhibits substantial processivity, enabling immediate and efficient DNA synthesis.
Profit: This brings about shorter reaction occasions and better yields, building the polymerase appropriate for demanding and time-sensitive projects.
3. Robust Overall performance
Overview: Phanta Max is formulated to deliver reputable overall performance throughout an array of templates and circumstances.
Gain: It assures consistent effects, even with intricate or GC-rich templates, improving the flexibility of the experiments.
four. Lengthy Fragment Amplification
Overview: Effective at amplifying lengthy DNA fragments as many as forty kb from genomic DNA.
Advantage: This means is particularly handy for applications necessitating the amplification of enormous genomic locations.
Applications
Polymerase Chain Reaction (PCR)
Use Scenario: Amplifying DNA for various downstream programs for instance cloning, sequencing, and analysis.
Advantage: The large-fidelity and effectiveness of Phanta Max assure exact and sturdy PCR benefits.
Cloning and Mutagenesis
Use Situation: Making precise DNA fragments for cloning into vectors or developing specific mutations.
Advantage: Minimizes the chance of errors in the course of amplification, delivering reliable constructs for further more experiments.
Subsequent-Generation Sequencing (NGS)
Use Situation: Getting ready libraries for NGS.
Advantage: Higher-fidelity amplification decreases the introduction of sequencing problems, increasing facts excellent.
Gene Synthesis
Use Scenario: Synthesizing prolonged and complicated genes for practical research or therapeutic applications.
Gain: The polymerase’s power to amplify very long DNA fragments makes certain the prosperous synthesis of enormous genes.
Protocol and Usage
one. Response Setup
Factors:
Template DNA: Commonly 10 ng for plasmid or 100 ng for genomic DNA.
Primers: 0.two µM Each and every.
dNTPs: 200 µM Just about every.
Phanta Max Super-Fidelity DNA Polymerase: one unit for every 50 µL response.
Buffer: Provided With all the enzyme, make sure good concentration According to producer’s Directions.
MgCl₂: A part of the buffer or additional independently if specified.
2. Thermal Biking Circumstances
Original Denaturation: 95°C for three minutes.
Denaturation: ninety five°C for 30 seconds.
Annealing: fifty five-sixty five°C for 30 seconds (improve according to primer Tm).
Extension: seventy two°C for 1 moment for every kb of goal.
Final Extension: 72°C for five minutes.
Cycle Quantity: Commonly 25-35 cycles, determined by template complexity and abundance.
3. Optimization Guidelines
Template High quality: Ensure the DNA template is of superior phanta max super-fidelity dna polymerase purity in order to avoid inhibitors that could have an impact on the polymerase’s overall performance.
Primer Layout: Use primers with a Tm in two-three°C of each other for ideal annealing.
Mg²⁺ Focus: Regulate Mg²⁺ focus if essential to enhance generate and specificity.
Summary
Phanta Max Super-Fidelity DNA Polymerase (P505) is an advanced Device for prime-precision DNA amplification. Its Tremendous-fidelity, performance, and strong overall performance allow it to be ideal for an array of molecular biology apps, making sure responsible and p505 accurate outcomes. Whether you are working on cloning, gene synthesis, or subsequent-generation sequencing, Phanta Max gives the precision and dependability essential to accomplish your exploration aims.